About this Medicine article

Quantitative Studies of Hydrodynamic Effects and Cross‐relaxation in Protein Solutions and Tissues with Proton and Deuteron Longitudinal Relaxation Times by Jianhui Zhong; John C. Gore; Ian M. Armitage is a Medicine article available to read on EtoBox.

## Abstract Longitudinal relaxation times __T__~1~ of water protons were measured in 5% protein solutions at different static magnetic fields (0.47, 2, and 7 T), for proteins with molecular weight ranging between 1.4 and 480 kDa and in solvents of varying degrees of deuteration. __T__~1~, values were also obtained for rat liver soaked with Krebs‐Ringer solutions of varying degrees of deuteration at the above fields. For the samples containing D~2~O, __T__~1~ for deuterium was also measured at fields 2 and 7 T. The deuterium measurements were used to estimate water rotational correlation times which were in turn used to estimate the contribution of so‐called “hydrodynamic effects” of macromolecules to proton relaxation. The proton relaxation rates at full deuteration were compared with those in protonated solvent (water) to obtain a second, direct measurement of this effect. Both measurements provide quantitation of the hydrodynamic effects, free from the contributions of other effects that are transparent to deuteration, and results from both measurements agree with each other reasonably well. The cross‐relaxation rate between solute and solvent protons, and the contribution of par

Author

Jianhui Zhong; John C. Gore; Ian M. Armitage

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0740-3194)

Published

1990

Language

EN

Field

Medicine (Health Sciences)