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Highly Sensitive Analysis of Methamphetamine and Amphetamine in Human Whole Blood Using Headspace Solid-phase Microextraction and Gas Chromatography–mass Spectrometry by Kazuo Okajima; Akira Namera; Mikio Yashiki; Ichiro Tsukue; Tohru Kojima is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A simple and highly sensitive method for analysis of derivatized methamphetamine (MA) and amphetamine (AM) in whole blood was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography±mass spectrometry electron impact ionization selected ion monitoring (GC±MS-EI-SIM). A whole blood sample, deuterated-MA (d 5 -MA), as an internal standard (IS), tri-n-propylamine and penta ̄uorobenzyl bromide were placed in a vial. The vial was heated and stirred at 908C for 30 min. Then the extraction ®ber of the SPME was exposed at 908C for 30 min in the headspace of the vial while being stirred. The derivatives adsorbed on the ®ber were desorbed by exposing the ®ber in the injection port of a GC±MS. The calibration curves showed linearity in the range of 0.5±1000 ng/g for both MA and AM. The time for analysis was about 80 min per sample. In addition, this proposed method was applied to two autopsy cases where MA ingestion was suspected. In one case, MA and AM concentrations in the mixed left and right heart blood were 165 and 36.9 ng/g, respectively. In the other case, MA and AM concentrations were 1.79 and 0.119 mg/g in the left heart blood, and 1.27 and 0.074 mg/g in

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Author

Kazuo Okajima; Akira Namera; Mikio Yashiki; Ichiro Tsukue; Tohru Kojima

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0379-0738)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)