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Can I read Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO~2~ nanofibrous surfaces on EtoBox?
Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO~2~ nanofibrous surfaces by Ai Wen Tan; Lelia Tay; Kien Hui Chua; Roslina Ahmad; Sheikh Ali Akbar; Belinda Pingguan-Murphy is a Materials Science article available to read on EtoBox.
What is Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO~2~ nanofibrous surfaces about?
Two important criteria of an ideal biomaterial in the field of stem cells research are to regulate the cell proliferation without the loss of its pluripotency and to direct the differentiation into a specific cell lineage when desired. The present study describes the influence of TiO2 nanofibrous surface structures on the regulation of proliferation and stemness preservation of adipose-derived stem cells (ADSCs). TiO2 nanofiber arrays were produced in situ onto Ti-6Al-4V substrate via a thermal oxidation process and the successful fabrication of these nanostructures was confirmed by field emission scanning electron microscopy (FESEM), energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), and contact angle measurement. ADSCs were seeded on two types of Ti-6Al-4V surfaces (TiO2 nanofibers and flat control), and their morphology, proliferation, and stemness expression were analyzed using FESEM, AlamarBlue assay, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) after 2 weeks of incubation, respectively. The results show that ADSCs exhibit better adhesion and significantly enhanced proliferation on the TiO2 nanofibrous surfaces compared to the f
Who reads Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO~2~ nanofibrous surfaces?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Ai Wen Tan; Lelia Tay; Kien Hui Chua; Roslina Ahmad; Sheikh Ali Akbar; Belinda Pingguan-Murphy
- Publisher
- Informa UK Limited
- Published
- 2014
- Language
- EN
- Field
- Materials Science (Physical Sciences)