Can I read Automated Latex Nephelometric Immunoassay for the Measurement of Serum Lipoprotein (A) on EtoBox?

Automated Latex Nephelometric Immunoassay for the Measurement of Serum Lipoprotein (A) by Dr. Luis Borque; Antonio Rus; Jorge del Cura; Carlos Maside; Jesus Escanero is a Medicine article available to read on EtoBox.

What is Automated Latex Nephelometric Immunoassay for the Measurement of Serum Lipoprotein (A) about?

A sensitive immunoassay based on latex particle agglutination has been developed for measuring lipoprotein Lp(a) concentrations in serum or plasma. Carboxylated latex particles (diameter 240 nm) covalently coated with F(ab'), fragments of anti-lipoprotein Lp(a) antibodies are incubated with diluted sample (400-fold) for 12 min at rmm temperature, with the resulting agglutination quantified by measuring the change of light-scatter produced. The assay has been automated on the Behring nephelometer analyzer with a sampling rate of 150 samplesihour. This assay generates a standard curve in the range of 27 to 1750 mg/L, showing inter-assay precision of less than 8%. There were no interferences from plasminogen, bilirubin, Intralipid'", haemoglobin, rheumatoid factor, and apolipoprotein B. No significant differences were observed when fresh and frozen samples were compared. Sample pretreatment with "Lipoclean" clearing agent and sample lyophillization decreased the agglutinating reaction. In two separate studies using 77 and 112 patient sera the Lp(a) values, determined by the latex nephelometric method, the Terumo Macra'" Lp(a) ELlSAtest, and the Pharmacia Apo(a) radioimmunoassay method

Who reads Automated Latex Nephelometric Immunoassay for the Measurement of Serum Lipoprotein (A)?

Author

Dr. Luis Borque; Antonio Rus; Jorge del Cura; Carlos Maside; Jesus Escanero

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0887-8013)

Published

1993

Language

EN

Field

Medicine (Health Sciences)