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Heparin Penetration Into and Permeation Through Human Skin from Aqueous and Liposomal Formulations in Vitro by Gabriele Betz; Pegah Nowbakht; Roger Imboden; Georgios Imanidis is a Pharmacology, Toxicology and Pharmaceutics article available to read on EtoBox.

What is Heparin Penetration Into and Permeation Through Human Skin from Aqueous and Liposomal Formulations in Vitro about?

The transport of unfractionated (UH) and low molecular weight Heparin (LMWH) in human skin was investigated in vitro using heat separated epidermal membrane and dermis and the effect of liposomal formulations with Phospholipon ® 80 (PL80) and Sphingomyelin (SM) was assessed. The distribution of Heparin within skin tissue was studied by the tape stripping method. Heparin concentrations were measured with a biological assay. Transepidermal water loss was determined to characterize barrier properties of skin. No consistent permeation of Heparin through epidermal membrane was detected. Penetration into the epidermal membrane was for LMWH significantly greater than for UH. Accumulation of UH was largely restricted to the outermost layers of the stratum corneum while LMWH penetrated into deeper epidermal layers. UH penetration into epidermis was detected for the PL80 liposomal formulation only. The extent of LMWH penetration was independent of the formulation, LMWH, however, showed a trend to accumulate in deeper epidermal layers for the PL80 compared to the aqueous formulation. Thus, molecular weight and liposomal formulations influenced the penetration pattern of Heparin in the epiderm

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Author

Gabriele Betz; Pegah Nowbakht; Roger Imboden; Georgios Imanidis

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-5173)

Published

2001

Language

EN

Field

Pharmacology, Toxicology and Pharmaceutics (Life Sciences)