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Raloxifene inhibits pancreatic adenocarcinoma growth by interfering with ERβ and IL-6/gp130/STAT3 signaling by Ioannis Pozios; Nina N. Seel; Nina A. Hering; Lisa Hartmann; Verena Liu; Peter Camaj; Mario H. Müller; Lucas D. Lee; Christiane J. Bruns; Martin E. Kreis; Hendrik Seeliger is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract ## Purpose Currently, the exact role of estrogen receptor (ER) signaling in pancreatic cancer is unknown. Recently, we showed that expression of phosphorylated ERβ correlates with a poor prognosis in patients with pancreatic ductal adenocarcinoma (PDAC). Here, we hypothesized that raloxifene, a FDA-approved selective ER modulator (SERM), may suppress PDAC tumor growth by interfering with ERβ signaling. To test this hypothesis, we studied the impact of raloxifene on interleukin-6/glycoprotein-130/signal transducer and activator of transcription-3 (IL-6/gp130/STAT3) signaling. ## Methods Human PDAC cell lines were exposed to raloxifene after which growth inhibition was assessed using a BrdU assay. ER knockdown was performed using siRNAs specific for ERα and ERβ. The effects of raloxifene on IL-6 expression and STAT3 phosphorylation in PDAC cells were assessed by ELISA and Western blotting, respectively. In addition, raloxifene was administered to an orthotopic PDAC tumor xenograft mouse model, after which tumor growth was monitored and immunohistochemistry was performed. ## Results Raloxifene inhibited the in vitro growth of PDAC cells, and this effect was reversed by siR

Who reads Raloxifene inhibits pancreatic adenocarcinoma growth by interfering with ERβ and IL-6/gp130/STAT3 signaling?

Author

Ioannis Pozios; Nina N. Seel; Nina A. Hering; Lisa Hartmann; Verena Liu; Peter Camaj; Mario H. Müller; Lucas D. Lee; Christiane J. Bruns; Martin E. Kreis; Hendrik Seeliger

Publisher

Springer Science and Business Media LLC

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)