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Loss of a neutralizing epitope by a spontaneous point mutation in the V3 loop of HIV-1 isolated from an infected laboratory worker. by F di Marzo Veronese; M S Reitz; G Gupta; M Robert-Guroff; C Boyer-Thompson; A Louie; R C Gallo; P Lusso is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The third hypervariable region, or V3 loop, represents the principal neutralizing domain of the gp120 envelope glycoprotein of human immunodeficiency virus type 1 (HN-1). Sequential viral isolates from a laboratory worker (LW) accidentally infected with HN-lIIIB in 1985 were analyzed using type-specific neutralizing monoclonal antibodies directed to the V3 loop. A single amino acid substitution, Ala + Thr at position 21 in the V3 loop of HW-lLw isolated in 1987, was shown to determine the loss of the neutralizing epitope recognized by one of the monoclonal antibodies (M77). However, this antibody efficiently recognized linear V3 loop peptides containing either the Ala or Thr residue at position 21, indicating that a local change in conformation was responsible for the epitope loss in the native gp120. Molecular modeling studies, experimentally supported by different amino acid replacements at position 21, indicated that the Ala .-\* Thr substitution leads to a drastic change in the do-~~ \* This work was supported in part by Contracts N01-CP-73723 and N01-CP-73722 from the National Cancer Institute. The costs of publication of this article were defrayed in part by the payment of pa

Who reads Loss of a neutralizing epitope by a spontaneous point mutation in the V3 loop of HIV-1 isolated from an infected laboratory worker.?

Author

F di Marzo Veronese; M S Reitz; G Gupta; M Robert-Guroff; C Boyer-Thompson; A Louie; R C Gallo; P Lusso

Publisher

Elsevier BV

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)