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Poly(methylene Co-guanidine) Coated Alginate as an Encapsulation Matrix for Urease by Elizabeth Hearn; Ronald J Neufeld is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Urease was encapsulated within alginate beads, coated with poly(methylene co-guanidine) membranes via polyelectrolyte complexation. Membrane thickness increased with reaction time to 53 mm after 80 min, and to 59 mm with an increase in co-guanidine concentration from 2.5 to 20 mg ml -1 . A 70% mass and 31% activity yield of urease resulted following encapsulation. Although co-guanidine strongly inhibited freely soluble urease (I 0.5 = 5.8 mg ml -1 co-guanidine), immobilization stabilized the enzyme against inactivation. Encapsulated activity declined as the polycation concentration used for membrane formation increased; however an activity loss of only 35% was observed when the co-guanidine concentration was as high as 5 mg ml -1 . Glucose protected against inactivation, with I 0.5 increasing to 28.5 mg ml -1 for the freely soluble enzyme. When the beads were coated with co-guanidine in the presence of glucose, encapsulated urease activity was fully retained.

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Author

Elizabeth Hearn; Ronald J Neufeld

Publisher

Elsevier Science; Elsevier ; Elsevier Applied Science; Elsevier BV (ISSN 1873-3298)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)