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Qian Yang Yu Yin Granule protects against hypertension-induced renal injury by epigenetic mechanism linked to Nicotinamide N-Methyltransferase (NNMT) expression by Shu-Fei Zhang; Xin-Jing Mao; Wei-Min Jiang; Zhu-Yuan Fang is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Ethnopharmacological relevance: Qian yang yu yin granule (qyyy) is a chinese herbal formulation. it is used to treat hypertensive nephropathy for decades in china, but it is unknown that the exact mechanism of qyyy on hypertensive nephropathy. ## Aims of study: The present study was to elucidate its epigenetic mechanism of qyyy on hypertensive nephropathy. ## Materials and methods: In the current study, hek293t cells' proliferation induced by ang ii was chosen to observe epigenetic mechanisms of qyyy on renal damage. the cell proliferation was examined by mtt assays and ethynyldeoxyuridine analysis. cell cycle analysis was performed. after treatment with qyyy, expression of nicotinamide n-methyltransferase (nnmt), sirtuin1(sirt1), s-adenosylhomocysteine(sah), histone h3k4 methylation, and cortactin acetylation(acetyl-cortactin,ac-cortactin) were further investigated by western-blotting and real time pcr. dna methylation was detected by elisa. the study also observed the changes of sirt1, sah, h3k4 methylation, acetyl-cortactin when nnmt over-expressed by lentivirus transfection. angiotensin ii(ang ii) induced renal damage in spontaneously hypertensive rats(shr). after eight week

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Author

Shu-Fei Zhang; Xin-Jing Mao; Wei-Min Jiang; Zhu-Yuan Fang

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-8741)

Published

2020

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)