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Can I read Phage-assisted Evolution and Protein Engineering Yield Compact, Efficient Prime Editors on EtoBox?
Phage-assisted Evolution and Protein Engineering Yield Compact, Efficient Prime Editors by Jordan L. Doman; Smriti Pandey; Monica E. Neugebauer; Meirui An; Jessie R. Davis; Peyton B. Randolph; Amber McElroy; Xin D. Gao; Aditya Raguram; Michelle F. Richter; Kelcee A. Everette; Samagya Banskota; Kathryn Tian; Y. Allen Tao; Jakub Tolar; Mark J. Osborn; David R. Liu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Phage-assisted Evolution and Protein Engineering Yield Compact, Efficient Prime Editors about?
## Summary Prime editing enables a wide variety of precise genome edits in living cells. Here we use protein evolution and engineering to generate prime editors with reduced size and improved efficiency. Using phage-assisted evolution, we improved editing efficiencies of compact reverse transcriptases by up to 22-fold and generated prime editors that are 516–810 base pairs smaller than the current-generation editor PEmax. We discovered that different reverse transcriptases specialize in different types of edits and used this insight to generate reverse transcriptases that outperform PEmax and PEmaxΔRNaseH, the truncated editor used in dual-AAV delivery systems. Finally, we generated Cas9 domains that improve prime editing. These resulting editors (PE6a-g) enhance therapeutically relevant editing in patient-derived fibroblasts and primary human T-cells. PE6 variants also enable longer insertions to be installed __in vivo__ following dual-AAV delivery, achieving 40% __loxP__ insertion in the cortex of the murine brain, a 24-fold improvement compared to previous state-of-the-art prime editors.
Who reads Phage-assisted Evolution and Protein Engineering Yield Compact, Efficient Prime Editors?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jordan L. Doman; Smriti Pandey; Monica E. Neugebauer; Meirui An; Jessie R. Davis; Peyton B. Randolph; Amber McElroy; Xin D. Gao; Aditya Raguram; Michelle F. Richter; Kelcee A. Everette; Samagya Banskota; Kathryn Tian; Y. Allen Tao; Jakub Tolar; Mark J. Osborn; David R. Liu
- Publisher
- Elsevier BV
- Published
- 2023
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)