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Antisense‐inhibition of ADP‐glucose pyrophosphorylase in developing seeds of Vicia narbonensis moderately decreases starch but increases protein content and affects seed maturation by Hans Weber; Hardy Rolletschek; Ute Heim; Sabine Golombek; Sabine Gubatz; Ulrich Wobus is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Summary The small subunit of a __Vicia faba__ ADP‐glucose pyrophosphorylase (AGP) cDNA was expressed in antisense orientation in __Vicia narbonensis__ under the control of the seed‐specific legumin B4 promoter. From several independent transgenic lines both ADP‐glucose pyrophosphorylase AGP‐mRNA and AGP enzyme activity were reduced by up to 95% in the cotyledons during the mid‐ to late‐maturation phase. Starch was moderately decreased and sucrose was increased. In two of three lines, transcripts encoding the large subunit of AGP and the storage protein vicilin were increased, whereas legumin B‐mRNA was decreased. Transcripts of other storage‐associated genes were not altered. The cotyledons contained more protein and total nitrogen. Despite the reduction in starch, total carbon was not decreased and dry weight was unchanged. Compared to the wild type, transgenic seeds contained more water and accumulated dry weight during a longer period, and therefore had a prolonged seed‐filling period. Transgenic cotyledon cells of comparable age to the wild type were more highly vacuolated and contained smaller starch grains, indicating a delay in cellular differentiation. We conclude that a

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Author

Hans Weber; Hardy Rolletschek; Ute Heim; Sabine Golombek; Sabine Gubatz; Ulrich Wobus

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

2000

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)