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Rapid Flow Injection Electrochemical Detection of 3,3′,4,4′ Tetrachlorobiphenyl Using Stabilized Lipid Membranes with Incorporated Sheep Antibody by Dimitrios Nikolelis; Nikolas Psaroudakis; Antonis Michaloliakos; Georgia-Paraskevi Nikoleli; Michael Scoullos is a Chemistry article available to read on EtoBox.
What is Rapid Flow Injection Electrochemical Detection of 3,3′,4,4′ Tetrachlorobiphenyl Using Stabilized Lipid Membranes with Incorporated Sheep Antibody about?
## Abstract An electrochemical biosensor based on a supported polymerized lipid film with incorporated sheep anti-3,3′,4,4′ tetrachlorobiphenyl (PCB congener 77) antibody using flow injection analysis was developed. The polymerized lipid film contained 85% (w/w) dipalmitoylphosphatidylcholine (DPPC) and 15% (w/w) dipalmitoylphosphatidic acid (DPPA), methacrylic acid, ethylene glycol dimethacrylate, AIBN and sheep anti-congener 77 antiserum. Congener 77 was injected into flowing carrier electrolyte and the flow stopped to detect the antigen. These membranes gave only a single transient proportional to log [congener 77] from 10−8 to 10−5 M, with a detection limit of ca. 10−8 M. A membrane containing 35% (w/w) DPPA was used to examine regeneration. The maximum number of cycles was about 5.
Who reads Rapid Flow Injection Electrochemical Detection of 3,3′,4,4′ Tetrachlorobiphenyl Using Stabilized Lipid Membranes with Incorporated Sheep Antibody?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Dimitrios Nikolelis; Nikolas Psaroudakis; Antonis Michaloliakos; Georgia-Paraskevi Nikoleli; Michael Scoullos
- Publisher
- Walter de Gruyter GmbH
- Published
- 2013
- Language
- EN
- Field
- Chemistry (Physical Sciences)