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Expression of CD13/aminopeptidase N and CD10/neutral endopeptidase on cultured human keratinocytes by Jelka Gabrilovac; Barbara Čupić; Davorka Breljak; Marija Zekušić; Milivoj Boranić is a Immunology and Microbiology article available to read on EtoBox.

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Keratinocytes actively participate in immune response and inflammation by secreting cytokines and chemokines. Membrane-bound peptidases serve as negative loop in controlling concentration of peptide signalling molecules. Recently, they have also been proposed as additional mechanism of cell-to-cell interaction and as signalling molecules. In this study, we examined expression of two membrane-bound peptidases: aminopeptidase N (APN; EC 3.4.11.2; CD13) and neutral endopeptidase (NEP; EC 3.4.24.11; CD10) on nonstimulated cultured human keratinocytes obtained from healthy skin. Membrane expression of CD13 and CD10 was analysed by FACS and fluorescent microscope. Functional properties of CD13 and CD10 were examined by testing their enzymatic activity towards selective substrates. The data were compared to those obtained on cultured nonstimulated human skin fibroblasts expressing both CD13/APN and CD10/NEP. Approximately one-third (i.e. 31.7 ± 2.8%; n = 3) of cultured keratinocyte express CD13 as compared to fibroblasts which are 100% CD13 + (n = 3). Density of CD13 on keratinocytes is several times lower than on fibroblasts. Membrane CD13 expression on keratinocytes was associated with

Who reads Expression of CD13/aminopeptidase N and CD10/neutral endopeptidase on cultured human keratinocytes?

Author

Jelka Gabrilovac; Barbara Čupić; Davorka Breljak; Marija Zekušić; Milivoj Boranić

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-2478)

Published

2004

Language

EN

Field

Immunology and Microbiology (Life Sciences)