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Interphase FISH assays for the detection of translocations with breakpoints in immunoglobulin light chain loci by José I. Martín‐Subero; Lana Harder; Stefan Gesk; Brigitte Schlegelberger; Werner Grote; José A. Martinez‐Climent; Martin J.S. Dyer; Francisco J. Novo; María J. Calasanz; Reiner Siebert is a Medicine article available to read on EtoBox.
What is Interphase FISH assays for the detection of translocations with breakpoints in immunoglobulin light chain loci about?
## Abstract Many B‐cell malignancies bear chromosomal translocations juxtaposing immunoglobulin (__IG__) genes with oncogenes, resulting in deregulated expression of the latter. Translocations affecting the __IG__ heavy chain (__IGH__) locus in chromosomal region 14q32 are most prevalent. However, variant translocations involving the __IG__ kappa (__IGK__) locus in 2p12 or the __IG__ lambda (__IGL__) locus in 22q11 occur recurrently in B‐cell neoplasias. No routine methods for the detection of all breakpoints involving __IG__ light chain loci independently of the translocation partner have been described. For this reason, we have designed 2 novel interphase fluorescence __in situ__ hybridization (FISH) assays using differentially labeled probes flanking the __IGK__ and __IGL__ locus, respectively. Based on extensive control studies, the diagnostic thresholds for the detection of breakpoints were set at 0.3% for __IGK__ and 1.4% for __IGL____.__ Fifteen cases of B‐cell malignancies with cytogenetically detectable chromosomal abnormalities in 2p11‐14 were investigated with the FISH assay for __IGK____.__ Breakpoints affecting the __IGK__ locus were detected in 7 cases including all 4
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- Author
- José I. Martín‐Subero; Lana Harder; Stefan Gesk; Brigitte Schlegelberger; Werner Grote; José A. Martinez‐Climent; Martin J.S. Dyer; Francisco J. Novo; María J. Calasanz; Reiner Siebert
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2002
- Language
- EN
- Field
- Medicine (Health Sciences)