Opening book details…
Can I read Evaluation of Mitochondrial Membrane Potential Using a Computerized Device with a Tetraphenylphosphonium-selective Electrode on EtoBox?
Evaluation of Mitochondrial Membrane Potential Using a Computerized Device with a Tetraphenylphosphonium-selective Electrode by Anna Labajova; Alena Vojtiskova; Pavla Krivakova; Jiri Kofranek; Zdenek Drahota; Josef Houstek is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Evaluation of Mitochondrial Membrane Potential Using a Computerized Device with a Tetraphenylphosphonium-selective Electrode about?
Mitochondrial membrane potential (Dw m ) plays important roles in the normal function of cells and in pathobiochemical situations. The application of ion-selective electrodes for the measurement of Dw m is important for studying normal biological reactions and pathways and mitochondrial diseases. We constructed and optimized a computerized device for real-time monitoring of the Dw m , which included modification of tetraphenylphosphonium (TPP + )-selective membrane that improved reproducibility of the TPP + -selective electrode. Application of MATLAB software increased the sensitivity of the system. We tested our improved device for membrane potential measurements of isolated mitochondria (in absolute scale of millivolts). In addition, we assessed relative changes of Dw m (as changes in TPP + concentration) of digitonin-permeabilized cells (hepatocytes, control transmitochondrial cybrids, HeLa G and BSC-40) after addition of substrates, inhibitors, and uncoupler of respiratory chain. Our system can be successfully used for studies of many aspects of the regulation of mitochondrial bioenergetics and as a diagnostic tool for mitochondrial oxidative phosphorylation disorders.
Who reads Evaluation of Mitochondrial Membrane Potential Using a Computerized Device with a Tetraphenylphosphonium-selective Electrode?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Anna Labajova; Alena Vojtiskova; Pavla Krivakova; Jiri Kofranek; Zdenek Drahota; Josef Houstek
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)