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Prime editing: Its systematic optimization and current applications in disease treatment and agricultural breeding by Xiaoxiao Yu; Guanzhong Huo; Jintai Yu; Huiyuan Li; Jun Li is a Materials Science article available to read on EtoBox.

What is Prime editing: Its systematic optimization and current applications in disease treatment and agricultural breeding about?

CRISPR/Cas-mediated genome-editing technology has accelerated the development of the life sciences. Prime editing has raised genome editing to a new level because it allows for all 12 types of base substitutions, targeted insertions and deletions, large DNA fragment integration, and even combinations of these edits without generating DNA double-strand breaks. This versatile and game-changing technology has successfully been applied to human cells and plants, and it currently plays important roles in basic research, gene therapy, and crop breeding. Although prime editing has substantially expanded the range of possibilities for genome editing, its efficiency requires improvement. In this review, we briefly introduce prime editing and highlight recent optimizations that have improved the efficiency of prime editors. We also describe how the dual-pegRNA strategy has expanded current editing capabilities, and we summarize the potential of prime editing in treating mammalian diseases and improving crop breeding. Finally, we discuss the limitations of current prime editors and future prospects for optimizing these editors.

Who reads Prime editing: Its systematic optimization and current applications in disease treatment and agricultural breeding?

Author

Xiaoxiao Yu; Guanzhong Huo; Jintai Yu; Huiyuan Li; Jun Li

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Materials Science (Physical Sciences)