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Expression of the Duffy antigen/receptor for chemokines (DARC) by the inflamed synovial endothelium by Angela M. Patterson; Hilary Siddall; Giselle Chamberlain; Lucy Gardner; Jim Middleton is a Medicine article available to read on EtoBox.
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## Abstract The expression of chemokine binding sites on the endothelial cells of venules in inflamed synovia was examined and whether the Duffy antigen/receptor for chemokines (DARC) was involved. __Insitu__ binding assays were performed to determine the expression of chemokine binding sites from rheumatoid (__n__=10) and non‐rheumatoid (__n__=10) synovia. The expression of DARC protein and mRNA was examined by immunohistochemistry and northern blotting. The involvement of DARC in chemokine binding was studied by incubating sections with blocking antibodies to DARC (Fy3and 6), to find out if these reduced ^125^I‐IL‐8 binding. Binding of radiolabelled chemokines IL‐8, RANTES, MCP‐1, but not MIP‐1α, was found on venular endothelial cells in inflamed synovia from both rheumatoid and non‐rheumatoid patients. Excess homologous unlabelled chemokine displaced binding and excess unlabelled RANTES could displace radiolabelled IL‐8 binding. DARC protein expression was demonstrated on venular endothelial cells in all samples and DARC mRNA could be detected in extracts from synovia. There was downregulation of DARC protein and mRNA in rheumatoid samples. Binding of IL‐8 to both rheumatoid and
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- Author
- Angela M. Patterson; Hilary Siddall; Giselle Chamberlain; Lucy Gardner; Jim Middleton
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)
- Published
- 2002
- Language
- EN
- Field
- Medicine (Health Sciences)