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DNA:RNA hybrid G-quadruplex formation upstream of transcription start site by Zhang, Jia-yu; Xia, Ye; Hao, Yu-hua; Tan, Zheng is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is DNA:RNA hybrid G-quadruplex formation upstream of transcription start site about?

Bioinformatic analysis reveals an enrichment of putative DNA:RNA hybrid G-quadruplex-forming sequences (PHQS) on both sides of the transcription start sites (TSSs) in the genome of warm-blooded animals, suggesting a positive selection of PHQSs in evolution and functional role of DNA:RNA hybrid G-quadruplexes (HQs) in transcription. The formation of HQs downstream of TSS in transcribed DNA has been documented under __in vitro__ conditions; however, it is still not known if such HQs can form at the upstream side of TSSs. In this study, we report that such HQs can form in transcription in DNA with two to three guanine tracts if RNA carrying the required number of G-tracts is supplied. We also show that the formation of such HQs is dependent on the negative supercoiling generated by RNA polymerases. These results suggest that HQs may also form at the upstream side of TSSs __in vivo__ and play a role in transcription since the two requirements are satisfied in cells.

Who reads DNA:RNA hybrid G-quadruplex formation upstream of transcription start site?

Author

Zhang, Jia-yu; Xia, Ye; Hao, Yu-hua; Tan, Zheng

Publisher

Springer Science and Business Media LLC; London: Nature Publishing Group; [London]: Springer Nature; Nature Publishing Group; Society for Mining, Metallurgy and Exploration Inc.; Research Square; Springer Nature; Island Press (ISSN 2045-2322)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)