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Fhit gene alterations in hepatocarcinogenesis induced by a choline-deficient L-amino acid–defined diet in rats by Toshifumi Tsujiuchi; Yasutaka Sasaki; Yasuo Oka; Yoichi Konishi; Masahiro Tsutsumi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Alterations of the fragile histidine triad (__Fhit__) gene were investigated in rat hepatocarcinogenesis induced by a choline‐deficient L‐amino acid–defined (CDAA) diet. Males of the F344 strain, 6 wk of age, were fed a CDAA diet, and subgroups were killed at 2, 4, 12, 20, and 75 wk after the beginning of the experiment. Fifteen hepatocellular carcinomas (HCCs) were noted in rats by the last time point; they were dissected free from the surrounding tissue. Normal control liver specimens were obtained from 6‐wk‐old rats. Total RNAs were extracted from whole livers of animals fed the CDAA diet for 2, 4, 12, and 20 wk and from HCCs, for assessment of aberrant transcription of the __Fhit__ gene by reverse transcription–polymerase chain reaction. Aberrant transcripts were detected in livers of rats fed the CDAA diet for 4, 12, and 20 wk, but not 2 wk, as well as in 11 of 15 HCCs (73.3%). Southern blot analysis showed a genomic DNA abnormality in one of seven informative HCCs (14.3%), while Western blot analysis showed reduction of Fhit protein expression in seven of nine HCCs (77.8%). No abnormal expression was evident in the livers after exposure to the CDAA diet for 2–20 w

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Author

Toshifumi Tsujiuchi; Yasutaka Sasaki; Yasuo Oka; Yoichi Konishi; Masahiro Tsutsumi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)