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Inositol Trisphosphate Receptor Gene Expression and Hormonal Regulation in Osteoblast-like Cell Lines and Primary Osteoblastic Cell Cultures by Keith L. Kirkwood; Rosemary Dziak; Peter G. Bradford is a Medicine article available to read on EtoBox.

What is Inositol Trisphosphate Receptor Gene Expression and Hormonal Regulation in Osteoblast-like Cell Lines and Primary Osteoblastic Cell Cultures about?

The inositol trisphosphate receptor (IP3R) is an intracellular calcium channel that mediates the cellular actions of a wide variety of hormones, growth factors, and cytokines. In osteoblastic cell cultures, many bone resorbing hormones increase phosphoinositide turnover, inositol trisphosphate production, mobilization of intracellular calcium, and the secretion of osteoclast recruitment and activating factors. In this study, the effects of 171lestradiol, 1,25-dihydroxyvitamin D 3 (1,25(OHhD3)' phorbol ester, and serum on IP3R mRNA levels were evaluated in osteogenic-osteosarcoma cells and in primary osteoblastic cultures derived from neonatal rat calvaria. Type-specific reverse transcription polymerase chain reaction (RT-PCR) indicated that all cell types evaluated (G-292, U-2 OS, Saos-z, MC3T3-E1, UMR-106, and calvarial osteoblastic cells) express IP3R mRNA type I; G-292, U-2 OS, MC3T3-E1, and calvarial osteoblastic cells also express type n IP3R mRNA; and UMR-106 and the calvarial osteoblastic cells express type ill IP3R mRNA. Northern blot and RT-PCR analyses of human G-292 osteosarcoma cells and rat calvarial osteoblastic cells showed that phorbol ester and serum increase IP3R

Who reads Inositol Trisphosphate Receptor Gene Expression and Hormonal Regulation in Osteoblast-like Cell Lines and Primary Osteoblastic Cell Cultures?

Author

Keith L. Kirkwood; Rosemary Dziak; Peter G. Bradford

Publisher

American Society for Bone and Mineral Research; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0884-0431)

Published

2010

Language

EN

Field

Medicine (Health Sciences)