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Stereoselective HPLC assay of donepezil enantiomers with UV detection and its application to pharmacokinetics in rats by Mahasen A. Radwan; Heba H. Abdine; Bushra T. Al-Quadeb; Hassan Y. Aboul-Enein; Kenichiro Nakashima is a Chemistry article available to read on EtoBox.
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This investigation describes a new precise, sensitive and accurate stereoselective HPLC method for the simultaneous determination of donepezil enantiomers in tablets and plasma with enough sensitivity to follow its pharmacokinetics in rats up to 12h after single oral dosing. Enantiomeric resolution was achieved on a cellulose tris (3,5-dimethylphenyl carbamate) column known as Chiralcel OD, with UV detection at 268 nm, and the mobile phase consisted of n-hexane, isopropanol and triethylamine (87:12.9:0.1). Using the chromatographic conditions described, donepezil enantiomers were well resolved with mean retention times of 12.8 and 16.3 min, respectively. Linear response (r > 0.994) was observed over the range of 0.05-2 microg/ml of donepezil enantiomers, with detection limit of 20 ng/ml. The mean relative standard deviation (R.S.D.%) of the results of within-day precision and accuracy of the drug were 0.05) between inter- and intra-day studies for each enantiomers which confirmed the reproducibility of the assay method. The mean extraction efficiency was 92.6-93.2% of the enantiomers. The proposed method was found to be suitable and accurate for the quantitative determination of
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- Author
- Mahasen A. Radwan; Heba H. Abdine; Bushra T. Al-Quadeb; Hassan Y. Aboul-Enein; Kenichiro Nakashima
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)
- Published
- 2006
- Language
- EN
- Field
- Chemistry (Physical Sciences)