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Can I read Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Sample Preparation Techniques Designed for Various Peptide and Protein Analytes on EtoBox?
Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Sample Preparation Techniques Designed for Various Peptide and Protein Analytes by Martin Kussmann; Eckhard Nordhoff; Henrik Rahbek-Nielsen; Sophie Haebel; Martin Rossel-Larsen; Lene Jakobsen; Johan Gobom; Ekatarina Mirgorodskaya; Anne Kroll-Kristensen; Lisbeth Palm‖; Peter Roepstorff is a Chemistry article available to read on EtoBox.
What is Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Sample Preparation Techniques Designed for Various Peptide and Protein Analytes about?
This study encompasses a collection of experiences with regard to numerous matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) sample preparation techniques in terms of their suitability for di †erent peptide and protein analytes. Variants of both established and new sample preparation techniques for the MALDI-MS analysis of peptides and proteins are described. The importance of matrix selection, matrix and analyte concentration, pH adjustment, crystallization conditions and the use of additives is evaluated. The tolerance of the di †erent sample preparations towards salts, bu †ers, synthetic polymers, detergents, denaturants and other contaminants, and also the inÑuence of the preparation methods on undesired amino acid side-chain oxidation, are investigated. Moreover, the performance of on-target tryptic digestion and on-target disulÐde reduction is shown and a microscale puriÐcation procedure is described. According to this study, there is no universally applicable sample preparation for a broad variety of analytes. Rather, it is necessary to speciÐcally adapt the sample preparation to the analyte properties.
Who reads Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Sample Preparation Techniques Designed for Various Peptide and Protein Analytes?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Martin Kussmann; Eckhard Nordhoff; Henrik Rahbek-Nielsen; Sophie Haebel; Martin Rossel-Larsen; Lene Jakobsen; Johan Gobom; Ekatarina Mirgorodskaya; Anne Kroll-Kristensen; Lisbeth Palm‖; Peter Roepstorff
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley-Liss Inc; John Wiley & Sons Inc.; Wiley; Heyden And Son (ISSN 1076-5174)
- Published
- 1997
- Language
- EN
- Field
- Chemistry (Physical Sciences)