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Highly Sensitive Determination of microRNA Using Target‐Primed and Branched Rolling‐Circle Amplification by Yongqiang Cheng; Xian Zhang; Zhengping Li; Xiaoxia Jiao; Yucong Wang; Yali Zhang is a Chemistry article available to read on EtoBox.
What is Highly Sensitive Determination of microRNA Using Target‐Primed and Branched Rolling‐Circle Amplification about?
## Abstract **One‐nucleotide differences** in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling‐circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined.magnified image
Who reads Highly Sensitive Determination of microRNA Using Target‐Primed and Branched Rolling‐Circle Amplification?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Yongqiang Cheng; Xian Zhang; Zhengping Li; Xiaoxia Jiao; Yucong Wang; Yali Zhang
- Publisher
- Wiley
- Published
- 2009
- Language
- EN
- Field
- Chemistry (Physical Sciences)
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