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Can I read Transgenic cell lines as a useful tool to study the biochemistry of down-regulation of an endogenous rice gene using a heterologous diamine-oxidase cDNA on EtoBox?
Transgenic cell lines as a useful tool to study the biochemistry of down-regulation of an endogenous rice gene using a heterologous diamine-oxidase cDNA by Ludovic Bassie; Matthiew Noury; Jean Pierre Wisniewski; Lola Topsom; Paul Christou; Teresa Capell is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Transgenic cell lines as a useful tool to study the biochemistry of down-regulation of an endogenous rice gene using a heterologous diamine-oxidase cDNA about?
Transgenic rice (Oryza sativa L.) cell lines engineered with the pea diamine oxidase cDNA in antisense orientation under the control of two different promoters were recovered using particle bombardment. Plasmids p35Sdaoa and pEdaoa contained the pea diamine oxidase cDNA driven by the CaMV35S and the pea ENOD12 nodulin promoter, respectively. Molecular analyses confirmed the stable integration of the transgene and active transcription (mRNA) with both promoters driving the gene of interest. We observed a 2-fold decrease in diamine oxidase activity (P < 0.01) in transformed callus lines expressing p35Sdaoa compared to wild-type or lines expressing the selectable marker gene alone (hpt). In cell lines transformed with pEdaoa, a 2.5-fold reduction in enzyme activity was measured (P < 0.01). Biochemical analysis of cellular polyamines from p35Sdaoa transformants indicated up to a 6-fold increase in putrescine levels (P < 0.001). Concentrations of higher polyamines also increased, with spermidine and spermine levels increasing 8-(P < 0.05) and 3.5-fold (P < 0.01) respectively. A maximum of 2.5-fold increase in putrescine (P < 0.001) and spermidine (P 0.05) was observed in spermine leve
Who reads Transgenic cell lines as a useful tool to study the biochemistry of down-regulation of an endogenous rice gene using a heterologous diamine-oxidase cDNA?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Ludovic Bassie; Matthiew Noury; Jean Pierre Wisniewski; Lola Topsom; Paul Christou; Teresa Capell
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0981-9428)
- Published
- 2000
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)