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Biochemical characterization of extremozyme L-asparaginase from Pseudomonas sp. PCH199 for therapeutics by Sanyukta Darnal; Vijeta Patial; Virender Kumar; Subhash Kumar; Vijay Kumar; Yogendra S. Padwad; Dharam Singh is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Biochemical characterization of extremozyme L-asparaginase from Pseudomonas sp. PCH199 for therapeutics about?
Abstract L-asparaginase (L-ASNase) from microbial sources is a commercially vital enzyme to treat acute lymphoblastic leukemia. However, the side effects associated with the commercial formulations of L-ASNases intrigued to explore for efficient and desired pharmacological enzymatic features. Here, we report the biochemical and cytotoxic evaluation of periplasmic L-ASNase of Pseudomonas sp. PCH199 isolated from the soil of Betula utilis, the Himalayan birch. L-ASNase production from wild-type PCH199 was enhanced by 2.2-fold using the Response Surface Methodology (RSM). Increased production of periplasmic L-ASNase was obtained using an optimized osmotic shock method followed by its purification. The purified L-ASNase was a monomer of 37.0 kDa with optimum activity at pH 8.5 and 60 °C. It also showed thermostability retaining 100.0% (200 min) and 90.0% (70 min) of the activity at 37 and 50 °C, respectively. The Km and Vmax values of the purified enzyme were 0.164 ± 0.009 mM and 54.78 ± 0.4 U/mg, respectively. L-ASNase was cytotoxic to the K562 blood cancer cell line (IC50 value 0.309 U/mL) within 24 h resulting in apoptotic nuclear morphological changes as examined by DAPI staining.
Who reads Biochemical characterization of extremozyme L-asparaginase from Pseudomonas sp. PCH199 for therapeutics?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Sanyukta Darnal; Vijeta Patial; Virender Kumar; Subhash Kumar; Vijay Kumar; Yogendra S. Padwad; Dharam Singh
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)