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Fast high-performance liquid chromatographic analysis of naphthodianthrones and phloroglucinols from Hypericum perforatum extracts by Ari Tolonen; Anja Hohtola; Jorma Jalonen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract __Hypericum perforatum__ L. (St. John's Wort) has been used in modern medicine for treatments of depression and neuralgic disorders. An HPLC method with photodiode array detection for the rapid determination of the major active compounds, naphthodianthrones and phloroglucinols, has been developed. The method permits the determination of hypericin, protohypericin, pseudohypericin, protopseudohypericin, hyperforin and adhyperforin in an extract in less than 5 min. Good linearity over the range 0.5–200 μg/mL for hyperforin and 0.02–100 μg/mL for hypericin was observed. Intra‐assay accuracy and precision varied from 0.1 to 17% within these ranges. Lower levels of quantitative determination were 2 μg/mL for hyperforin and 0.5 μg/mL for hypericin, while detection limits were 0.1 and 0.02 μg/mL, respectively. Copyright © 2003 John Wiley & Sons, Ltd.

Who reads Fast high-performance liquid chromatographic analysis of naphthodianthrones and phloroglucinols from Hypericum perforatum extracts?

Author

Ari Tolonen; Anja Hohtola; Jorma Jalonen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0958-0344)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)