Can I read Quantitative Analysis of Intercellular Adhesive Specificity in Freshly Explanted and Cultured Cells on EtoBox?

Quantitative Analysis of Intercellular Adhesive Specificity in Freshly Explanted and Cultured Cells by F Sieber; S Roseman is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Quantitative Analysis of Intercellular Adhesive Specificity in Freshly Explanted and Cultured Cells about?

A new method is presented for the quantitative analysis of intercellular adhesive specificity. In this assay, two cell types are mixed, one unlabeled and the other labeled with the fluorescent dye, fluorescamine [4-phenylspiro(feran-2[3H],1'-phthalan)-3,3'-dione]. The resulting aggregates are analyzed by fluorescence microscopy to determine the number of labeled and unlabeled cells per aggregate. Random (nonspecific) aggregation was characterized by a binomial distribution, and adhesive specificity was accordingly quantified by the deviation (as determined by a chi-square test) from the calculated binomial distribution. The labeling procedure was simple and rapid, and experiments with 18 different cell types showed that it did not affect cell viability, morphology, rate and extent of adhesion, plating efficiency, and the capability of myogenic cells to undergo terminal differentiation. Most important, assays with morphologically identifiable cell pairs indicated that the fluorescent label neither induced apparent nor destroyed existing adhesive specificity. The most pronounced adhesive specificities were observed with freshly explanted cells from adult tissues and also with mixture

Who reads Quantitative Analysis of Intercellular Adhesive Specificity in Freshly Explanted and Cultured Cells?

Author

F Sieber; S Roseman

Publisher

The Rockefeller University Press

Published

1981

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)