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Analysis of Aminoglycoside Residues in Bovine Milk by Liquid Chromatography Electrospray Ion Trap Mass Spectrometry After Derivatization with Phenyl Isocyanate by Sherri B. Turnipseed; Susan B. Clark; Christine M. Karbiwnyk; Wendy C. Andersen; Keith E. Miller; Mark R. Madson is a Chemistry article available to read on EtoBox.
What is Analysis of Aminoglycoside Residues in Bovine Milk by Liquid Chromatography Electrospray Ion Trap Mass Spectrometry After Derivatization with Phenyl Isocyanate about?
A derivatization procedure using phenyl isocyanate was adapted to liquid chromatography ion trap mass spectrometry (LC-MS(n)) for confirmation and quantification of aminoglycoside residues in milk. Aminoglycoside residues were extracted from milk with acid and isolated from the matrix with a weak cation exchange solid-phase extraction cartridge. After isolating the compounds from the milk, derivatives of gentamicin, neomycin, and tobramycin were formed by reacting the drugs with phenyl isocyanate in the presence of triethylamine. The analytes were separated using a dilute formic acid/acetonitrile gradient on a reversed-phase LC column. The derivatized compounds were analyzed using positive ion electrospray LC-MS(n) with ion trap detection. Product ion spectra were generated from the derivatized protonated molecules. Specific ion transitions were evaluated for quantitative determination and qualitative confirmation of residues in milk. Using this procedure, residues were qualitatively confirmed in milk samples fortified with gentamicin and neomycin at levels ranging from 15 to 300 ng mL(-1). Gentamicin has four major components that were successfully separated and confirmed independ
Who reads Analysis of Aminoglycoside Residues in Bovine Milk by Liquid Chromatography Electrospray Ion Trap Mass Spectrometry After Derivatization with Phenyl Isocyanate?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Sherri B. Turnipseed; Susan B. Clark; Christine M. Karbiwnyk; Wendy C. Andersen; Keith E. Miller; Mark R. Madson
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)
- Published
- 2009
- Language
- EN
- Field
- Chemistry (Physical Sciences)