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274 Early life antibiotic exposure and genetic risk in neurodevelopmental disorders: effects on neurogenesis, the gut microbiome, and behavior by Courtney R. McDermott; Anya Mirmaglesi; Zhan Gao; Katherine Kimbark; Christiana Ntim; Xuesong Zhang; Xiaofeng Zhou; James H. Millonig; Emanuel DiCicco-Bloom is a Medicine article available to read on EtoBox.

What is 274 Early life antibiotic exposure and genetic risk in neurodevelopmental disorders: effects on neurogenesis, the gut microbiome, and behavior about?

OBJECTIVES/GOALS: Our long-term goal is to understand how both genetic and environmental (GxE) factors contribute to neurodevelopmental disorders (NDDs) so that we may potentially intervene in disease pathogenesis and design therapies to address functional deficiencies. METHODS/STUDY POPULATION: Our studies use a novel GxE model to determine how cephalosporin antibiotic exposure alters the gut microbiome, hippocampal neurogenesis, and behavior in the genetically vulnerable 16p11.2 microdeletion (16pDel) mouse. This mouse models one of the most frequently observed genetic risk variants implicated in NDDs, including ~1% of autism diagnoses. Wildtype and 16pDel littermates were exposed to saline or the cephalosporin, cefdinir, from postnatal days 5-9. We quantified changes in gut microbiota composition using 16S rRNA gene sequencing and utilized immunoblotting, immunohistochemistry, and bulk RNA gene sequencing to assess changes in hippocampal neurogenesis. An additional cohort of saline or cefdinir-exposed mice were subjected to a behavioral battery to assess changes in sociability and anxiety. RESULTS/ANTICIPATED RESULTS: We leveraged the next-generation microbiome bioinformatics pl

Who reads 274 Early life antibiotic exposure and genetic risk in neurodevelopmental disorders: effects on neurogenesis, the gut microbiome, and behavior?

Author

Courtney R. McDermott; Anya Mirmaglesi; Zhan Gao; Katherine Kimbark; Christiana Ntim; Xuesong Zhang; Xiaofeng Zhou; James H. Millonig; Emanuel DiCicco-Bloom

Publisher

Cambridge University Press

Published

2023

Language

EN

Field

Medicine (Health Sciences)