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Can I read Some Aspects of the Chromogen 3,3 ́,5,5 ́-Tetramethylbenzidine as Hydrogen Donor in a Horseradish Peroxidase Assay on EtoBox?
Some Aspects of the Chromogen 3,3 ́,5,5 ́-Tetramethylbenzidine as Hydrogen Donor in a Horseradish Peroxidase Assay by R. W. Bally; T. C. J. Gribnau is a Medicine article available to read on EtoBox.
What is Some Aspects of the Chromogen 3,3 ́,5,5 ́-Tetramethylbenzidine as Hydrogen Donor in a Horseradish Peroxidase Assay about?
Enzyme immunoassays frequently incorporate the use of horseradish perosidase äs the enzyme label. This enzyme usually catalyses the oxidation of a ehromogen which can be quantified after termination of the enzyme reaction. A chromogen widely used for this purpose is S^'^S'-tetramethylbenzidine. The two electron oxidation of tetramethylbenzidine yields a component with an absorbance maximum at 450 nm. If the enzyme reaction is terminated by lowering of the pH (< 1.0), an additional increase of the absorbance at 450 nm is observed. It is shown that this additional increase is partly due to a l .4-fold increase in the molar lineic absorbance of oxidized tetramethylbenzidine, caused by the acidic pH, äs well äs a quantitative shift of the existing equilibrium between tetramethylbenzidine, oxidized tetramethylbenzidine and their charge-transfer complex. The total absorbance increase upon acidification of the reaction mixture depends therefore on the reaction conditions äs well äs the reaction coordinate.
Who reads Some Aspects of the Chromogen 3,3 ́,5,5 ́-Tetramethylbenzidine as Hydrogen Donor in a Horseradish Peroxidase Assay?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- R. W. Bally; T. C. J. Gribnau
- Published
- 1989
- Language
- EN
- Field
- Medicine (Health Sciences)