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Single-Copy Transgenic Mice Method by wang2022pharm is a document available to read on EtoBox.

This report presents an efficient method for generating single-copy transgenic mice through site-specific integration in embryonic stem cells using FLPe recombinase. The strategy allows for the targeted insertion of tetracycline-inducible transgenes, ensuring predictable gene expression patterns in both stem cells and derived mice. The method demonstrates high targeting efficiency and the ability to analyze gene function without extensive breeding or screening of transgenic offspring.

Author

wang2022pharm

Language

EN