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A BACH2-BCL2L1 fusion gene resulting from a t(6;20)(q15;q11.2) chromosomal translocation in the lymphoma cell line BLUE-1 by Seval Türkmen; Mathias Riehn; Eva Klopocki; Mara Molkentin; Richard Reinhardt; Thomas Burmeister is a Medicine article available to read on EtoBox.

What is A BACH2-BCL2L1 fusion gene resulting from a t(6;20)(q15;q11.2) chromosomal translocation in the lymphoma cell line BLUE-1 about?

## Abstract Abnormalities of the long arm of chromosome 6 are a common feature in various B‐cell malignancies. In most cases, the genes involved have not yet been clearly identified. We have molecularly characterized the recently established Burkitt lymphoma cell line BLUE‐1 that carries a t(6;20)(q15;q11.2) rearrangement in addition to the typical t(8;14) with __MYC__‐__IGH__ fusion. To identify the gene loci involved on both chromosomes we applied a sequential BAC clone mapping strategy. By using RT‐PCR we were finally able to detect a chimeric mRNA transcript showing a fusion of the first (non‐coding) exon of __BACH2__ (BTB and CNC homology 1, basic leucine zipper transcription factor 2) on 6q15 to the second exon of __BCL2L1__ (__BCL‐X__) on 20q11. Various fusion transcripts were detected for different __BCL2L1__ (__BCL‐XL__) isoforms. The fusion ultimately results in strong expression of the BCL2L1 (BCL‐XL) anti‐apoptosis protein, as demonstrated by immunoblotting. This is the first report that shows the involvement of both __BCL2L1__ and the transcription factor __BACH2__ in a chromosomal rearrangement. It points to __BACH2__ as a possibly important target in lymphomas with 6

Who reads A BACH2-BCL2L1 fusion gene resulting from a t(6;20)(q15;q11.2) chromosomal translocation in the lymphoma cell line BLUE-1?

Author

Seval Türkmen; Mathias Riehn; Eva Klopocki; Mara Molkentin; Richard Reinhardt; Thomas Burmeister

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 1045-2257)

Published

2011

Language

EN

Field

Medicine (Life Sciences)