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Developmental anomalies and neoplasia in animals and cells deficient in the large zinc finger protein KRC by Carl E. Allen; Natarajan Muthusamy; Steven E. Weisbrode; Joung Woo Hong; Lai-Chu Wu is a Medicine article available to read on EtoBox.

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## Abstract The large zinc finger protein KRC binds to the signal sequences of V(D)J recombination and the κB motif. Disruption of KRC expression in cell lines resulted in increased cell proliferation, anchorage independence of growth, and uncoupling of nuclear division and cell division. In this report, the function of KRC was studied in a RAG2‐deficient blastocyst complementation animal model. KRC‐deficient embryonic stem cells were generated by homologous recombination and were introduced into __RAG2__^__−/−__^ blastocysts to generate __KRC__^__−/−__^__;RAG2__^__−/−__^ chimeric mice. The lymphoid compartments of chimeras examined at 5 weeks of age were developed, suggesting that KRC is not essential for V(D)J recombination development. However, by 6 months of age, there was a marked deficit in CD4^+^CD8^+^ thymocytes in the chimeras, suggesting that KRC may be involved in T‐lymphocyte survival. Additionally, one chimera developed anomalies, including postaxial polydactyly, hydronephrosis, and an extragonadal malignant teratoma. DNA analysis showed that the teratoma was derived from __KRC__^__−/−__^ embryonic stem cells. The teratoma had compound tissue organization and was infil

Who reads Developmental anomalies and neoplasia in animals and cells deficient in the large zinc finger protein KRC?

Author

Carl E. Allen; Natarajan Muthusamy; Steven E. Weisbrode; Joung Woo Hong; Lai-Chu Wu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 1045-2257)

Published

2002

Language

EN

Field

Medicine (Life Sciences)