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Can I read Direct labeling microRNA with an electrocatalytic moiety and its application in ultrasensitive microRNA assays on EtoBox?
Direct labeling microRNA with an electrocatalytic moiety and its application in ultrasensitive microRNA assays by Zhiqiang Gao; Yuan Hong Yu is a Engineering article available to read on EtoBox.
What is Direct labeling microRNA with an electrocatalytic moiety and its application in ultrasensitive microRNA assays about?
An ultrasensitive procedure for the detection of microRNA (miRNA) in total RNA is described in this work. The miRNA is directly labeled with a redox active and electrocatalytic moiety, Ru(PD) 2 Cl 2 (PD = 1,10-phenanthroline-5,6-dione), through coordinative bonds with purine bases in the miRNA molecule. The excellent electrocatalytic activity of the Ru(PD) 2 Cl 2 towards the oxidation of hydrazine makes it possible to conduct ultrasensitive miRNA detection. Under optimized experimental conditions, the assay allows the detection of miRNAs in the range of 0.50-400 pM with a detection limit of 0.20 pM in 2.5 l (0.50 amole). MicroRNA quantitation is therefore performed in as little as 10 ng of total RNA, providing a much-needed platform for miRNA expression analysis.
Who reads Direct labeling microRNA with an electrocatalytic moiety and its application in ultrasensitive microRNA assays?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Zhiqiang Gao; Yuan Hong Yu
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)
- Published
- 2006
- Language
- EN
- Field
- Engineering (Physical Sciences)