Can I read Blotting Intact Immunoglobulins and Other High-molecular-weight Proteins After Composite Agarose-polyacrylamide Gel Electrophoresis on EtoBox?

Blotting Intact Immunoglobulins and Other High-molecular-weight Proteins After Composite Agarose-polyacrylamide Gel Electrophoresis by Keith B. Elkon; Peter W. Jankowski; Jia-Li Chu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Blotting Intact Immunoglobulins and Other High-molecular-weight Proteins After Composite Agarose-polyacrylamide Gel Electrophoresis about?

A composite agarose-polyacrylamide gel containing urea and sodium dodecyl sulfate reliably resolved unreduced human immunoglobulins according to their molecular weight. Intact immunoglobulins and a number of other macromolecules were readily transferred to nitrocellulose paper by either capillary or electrophoretic blotting, although the latter technique was more effective. Conventional antigen probing as well as immobilized antibody studies can be performed on the nitrocellulose transfers.

Who reads Blotting Intact Immunoglobulins and Other High-molecular-weight Proteins After Composite Agarose-polyacrylamide Gel Electrophoresis?

Author

Keith B. Elkon; Peter W. Jankowski; Jia-Li Chu

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1984

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)