Can I read Mineralized Nodule Formation in Primary Osteoblasts Culture in Titanium Doped Phosphate Glass and In-house Prepared Freeze Dried Demineralized Bone Extracts on EtoBox?
Mineralized Nodule Formation in Primary Osteoblasts Culture in Titanium Doped Phosphate Glass and In-house Prepared Freeze Dried Demineralized Bone Extracts by Ensanya A. Abou Neel; Aghila Rani KG; A.R. Samsudin is a Materials Science article available to read on EtoBox.
What is Mineralized Nodule Formation in Primary Osteoblasts Culture in Titanium Doped Phosphate Glass and In-house Prepared Freeze Dried Demineralized Bone Extracts about?
Mineral trioxide aggregate (MTA) is widely used in dentistry as root perforation repair material, however due to its high alkalinity, tissue necrosis might occur. This study aimed to investigate the mineralization potential of titanium dioxide doped phosphate glass (TDPG) and in-house prepared freeze dried demineralized bone (FDDB) as substitutes for MTA. Methods: TDPG and FDDB were prepared and characterized using scanning electron microscopy coupled with energy dispersive X-ray and Raman spectroscopy. The extract of FDDB, TDPG and FDDB + TDPG was used for culture of primary human osteoblast cells; cells grown in the presence of tissue culture medium was used as control. Mineral trioxide aggregate (ProRoot® MTA White) was used as a control material. Cell viability was evaluated up to 72 h using XTT assay. The mineralization potential was assessed in the presence of osteogenic and non-osteogenic media using Alizarin Red staining after 7 and 14 days. Cell morphology was captured using phase contrast microscopy. Results: All tested materials showed irregular-shaped morphology with variation in particle sizes. Both TDPG and FDDB contain calcium, phosphorus, and oxygen as main elements
Who reads Mineralized Nodule Formation in Primary Osteoblasts Culture in Titanium Doped Phosphate Glass and In-house Prepared Freeze Dried Demineralized Bone Extracts?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Ensanya A. Abou Neel; Aghila Rani KG; A.R. Samsudin
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Materials Science (Physical Sciences)