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Genome analysis of the moss Physcomitrella patens (Hedw.) B.S.G. by Ralf Reski; Merle Faust; Xiao-Hui Wang; Michael Wehe; Wolfgang O. Abel is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A wild-type (WT) strain of the moss Physcomitrella patens (Hedw.) B.S.G., two mutants derived from it (PC22 and P24), and a somatic hybrid, PC22(+)P24, were analysed. Staining of metaphases revealed 54 +/- 2 chromosomes in the somatic hybrid and 27 chromosomes in the wild type and the two mutants. Using flow cytometry (FCM), DNA contents were calculated to be 0.6 pg (WT, PC22), 1.2 pg (P24), and 1.6 pg (PC22(+)P24) per nucleus, respectively. Southern hybridization provided evidence for at least one family of highly repetitive DNA and, furthermore, revealed different amounts of repetitive DNA in the four genotypes. However, these sequences cannot account for the 100% increase in the nuclear DNA amount in mutant P24, relative to wild type. In FCM analyses every moss genotype generated just one single peak of fluorescence, indicating an arrest in the cell cycle during the daytime. Thermal denaturation of wild-type DNA revealed a G+C content of 34.6% for total DNA and 38.6% for plastid DNA. A cDNA library of 1.2 x 10(6) independent clones was established, from which sequences homologous to cab and rbcS, respectively, were isolated. These genes show significant homologies to those of hi

Who reads Genome analysis of the moss Physcomitrella patens (Hedw.) B.S.G.?

Author

Ralf Reski; Merle Faust; Xiao-Hui Wang; Michael Wehe; Wolfgang O. Abel

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)