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Agrobacterium-mediated transformation of commercial melon (Cucumis melo L., cv. Amarillo Oro) by M. P. Vallés; J. M. Lasa is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Agrobacterium-mediated transformation of commercial melon (Cucumis melo L., cv. Amarillo Oro) about?

Cotyledon explants of muskmelon (Cucumis melo L., cv. Amarillo Oro) seedlings were co-cultivated with disarmed Agrobacterium tumefaciens strain LBA4404 that contained the binary vector plasmid pBI121.1. The T-DNA region of this binary vector contains the Nopaline synthase/neomycin phosphotransferase II (NPTII) chimeric gene for kanamycin resistance and the Cauliflower Mosaic Virus 35S/β-glucuronidase (GUS) chimeric gene. After infection, the cotyledon pieces were placed in induction medium containing 100 mg/l kanamycin. Putative transformed shoots were obtained, followed by the development of morphologically normal plantlets. The transgenic nature of regenerants was demonstrated by polymerase chain reaction, Southern blot analysis, plant growth on medium selective for the transgene (NPTII) and expression of the co-transformed GUS gene. Factors affecting the transformation procedure are discussed.

Who reads Agrobacterium-mediated transformation of commercial melon (Cucumis melo L., cv. Amarillo Oro)?

Author

M. P. Vallés; J. M. Lasa

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0721-7714)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)