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Two Types of Calcium Currents of the Mouse Bipolar Cells Recorded in the Retinal Slice Preparation by Pedro De La Villa; Cecilia F. Vaquero; Akimichi Kaneko is a Neuroscience article available to read on EtoBox.

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## Abstract In the vertebrate retina, the bipolar cell makes reciprocal synapses with amacrine cells at the axon terminal. It has been postulated that amacrine cells may control the transmitter release from bipolar cells by modulating their calcium currents (__I__~Ca~). To clarify this possibility calcium currents were studied in bipolar cells of the mouse retina using a slice preparation. __I__~Ca~ was identified by voltage clamp protocols, ionic substitution and pharmacological tools. Depolarization to –30 mV from a holding voltage of –80 mV induced an inward current consisting of an initial transient and a long‐lasting sustained component. The transient component was inactivated by holding the membrane at more positive voltages. Addition of 100 μm nifedipine suppressed the sustained component, leaving the transient component almost intact. The sustained component was enhanced when external solution contained 0.1 μm Bay K 8644 or when the external Ca^2+^ was substituted by equimolar Ba^2+^. Omega‐conotoxin (10 μmω‐ctxn GVIA) did not alter either component. We concluded that the transient component is a low‐voltage activated T‐type __I__~Ca~, while the sustained component is a hig

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Author

Pedro De La Villa; Cecilia F. Vaquero; Akimichi Kaneko

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-816X)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)