Can I read A fluorescence polarization immunoassay for cadmium(II) on EtoBox?

A fluorescence polarization immunoassay for cadmium(II) by David K Johnson is a Chemistry article available to read on EtoBox.

What is A fluorescence polarization immunoassay for cadmium(II) about?

A target for antibody production was prepared by a 1 : 1 reaction of the bicyclic dianhydride of diethylenetriamine-N,N, N ,N ,N -pentaacetic acid (DTPA) with (Z)-2-amino-␣-(1-tert-butoxycarbonyl)-1-methylethoxyimino)-4-thiazoleacetic acid to give a reactive intermediate that was used to derivatize (a) bovine serum albumin (BSA), (b) fluoresceinamine and (c) n-butylamine. The BSA conjugate was complexed with cadmium(II) and used as an immunogen to produce polyclonal antibodies in rabbits. The fluorescein conjugate was complexed with cadmium(II) to provide a fluorescent analog of the immunizing chelate structure, while the butylamine derivative was used to convert ionic cadmium(II) into a form recognized by the antibody. The immunogen proved to be sufficiently stable in vivo produce antibodies that selectively bound to the cadmium(II) chelate and the chelate structure in the fluorescein and butylamine derivatives successfully mirrored that against which the antibody had been raised, enabling the displacement of tracer from the antibody binding site to be monitored by fluorescence polarization. Competitive binding fluorescence polarization immunoassay (FPIA) standard curves were cons

Who reads A fluorescence polarization immunoassay for cadmium(II)?

Author

David K Johnson

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)

Published

1999

Field

Chemistry (Physical Sciences)