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Developmentally non-redundant SET domain proteins SUVH2 and SUVH9 are required for transcriptional gene silencing in Arabidopsis thaliana by Markus Kuhlmann; Michael Florian Mette is a Agricultural and Biological Sciences article available to read on EtoBox.
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In plants, RNA-directed DNA methylation (RdDM) and related transcriptional gene silencing (TGS) involve members of the suppressor of variegation 3-9-homologous (SUVH) group of putative histone methyltransferases. Utilizing a reverse genetic approach in __Arabidopsis thaliana__, we demonstrate that two closely related __SUVH__ members, __SUVH2__ and __SUVH9__, act partially non-redundant in RdDM. DNA methylation, transcript accumulation and association with histone modifications were analyzed at the endogenous RdDM target __AtSN1__ (a SINE-like retroelement) in __suvh2__ and __suvh9__ single as well as __suvh2__ __suvh9__ double mutants. __SUVH2__ was found to be required for full DNA methylation at __AtSN1__ in early seed development and was also higher expressed in seeds than at later developmental stages. __SUVH9__ had its impact on RdDM later during vegetative development of the plant and was also higher expressed during that stage than at earlier developmental stages. The strongest reduction of RdDM at __AtSN1__ was found in __suvh2__ __suvh9__ double mutant plants. Histone 3-lysine 9-dimethylation (H3K9me2) associated with __AtSN1__ was reduced only in the simultaneous absence
Who reads Developmentally non-redundant SET domain proteins SUVH2 and SUVH9 are required for transcriptional gene silencing in Arabidopsis thaliana?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Markus Kuhlmann; Michael Florian Mette
- Publisher
- Springer Science and Business Media LLC
- Published
- 2012
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)