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Enhanced function of cultured epithelium by genetic modification: Cell-based synthesis and delivery of growth factors by Sabine A. Eming; Martin L. Yarmush; Jeffrey R. Morgan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Skin substitutes, containing cultured keratinocytes of the epidermis (autologous or allogeneic cells), have been used in the treatment of severe burns and other defects of the skin such as chronic ulcers. Our goal is to enhance the functions of the cells used in these skin substitutes by genetic modification. We propose t o develop a genetically modified skin graft which would function as a cellbased vehicle for the local synthesis and delivery of wound-healing growth factors. Using retroviral-mediated gene transfer, we have introduced stable copies of the genes encoding platelet-derived growth factor (PDGF-A) or insulin-like growth factor-I (IGF-1) into cultured human diploid keratinocytes. After stable integration of these genes, the cells secreted significant levels of these growth factors, 744 ng and 502 ng/107 cells/24 h for PDGF-A and IGF-1, respectively. The modified cells were grown to confluence, detached as a multicell-layered epithelial sheet, and transplanted to athymic mice. Seven days after transplantation, grafts secreting PDGF-A or IGF-1 differentiated into a stratified epithelium comparable to unmodified cells. Most importantly, the newly synthesized connective tis

Who reads Enhanced function of cultured epithelium by genetic modification: Cell-based synthesis and delivery of growth factors?

Author

Sabine A. Eming; Martin L. Yarmush; Jeffrey R. Morgan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)