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Effects of Glutathione Depletion on Selenite- and Selenate-induced Embryotoxicity in Cultured Rat Embryos by Makoto Usami; Hirobumi Tabata; Yasuo Ohno is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Effects of depletion of reduced glutathione (GSH) on selenium (Se) embryotoxicity in cultured rat embryos were examined. Rat embryos at day 9.5 of gestation were cultured for 48 h in the presence of Se as either sodium selenite at 10 and 20 μM or sodium selenate at 30 and 100 μM. Embryonic GSH was depleted by the addition of 0.1 mM of L-buthionine-[S,R]-sulfoximine (BSO) without embryotoxicity, i.e., significant growth retardation and malformation of the embryos. Selenite at 10 μM or selenate at 100 μM significantly increased the incidence of malformation of the embryos. The incidence of selenite-induced malformation of the embryos at 20 μM was significantly decreased with BSO. On the contrary, the incidence of selenate-induced malformation at 30 μM was significantly increased with BSO. It was noted that the major malformed regions of the embryos by the embryotoxic concentration of BSO alone were the same to those affected by selenite or selenate. It was considered from these results that embryonic GSH was involved in the embryotoxicity of selenite and selenate. The embryotoxicity of selenate may not be mediated through the reduction to selenite. It was suggested that the formation

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Author

Makoto Usami; Hirobumi Tabata; Yasuo Ohno

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-3211)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)