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Novel Antioxidant Therapy with the Immediate Precursor to Glutathione, Î3-Glutamylcysteine (GGC), Ameliorates LPS-Induced Cellular Stress in In Vitro 3D-Differentiated Airway Model from Primary Cystic Fibrosis Human Bronchial Cells by Hewson, Chris K.; Capraro, Alexander; Wong, Sharon L.; Pandzic, Elvis; Zhong, Ling; Fernando, Bentotage S. M.; Awatade, Nikhil T.; Hart-Smith, Gene; Whan, Renee M.; Thomas, Shane R.; Jaffe, Adam; Bridge, Wallace J.; Waters, Shafagh A. is a Medicine article available to read on EtoBox.

What is Novel Antioxidant Therapy with the Immediate Precursor to Glutathione, Î3-Glutamylcysteine (GGC), Ameliorates LPS-Induced Cellular Stress in In Vitro 3D-Differentiated Airway Model from Primary Cystic Fibrosis Human Bronchial Cells about?

Systemic glutathione deficiency, inflammation, and oxidative stress are hallmarks of cystic fibrosis (CF), an inherited disease that causes persistent lung infections and severe damage to the respiratory system and many of the body organs. Improvements to current antioxidant therapeutic strategies are needed. The dietary supplement, γ-glutamylcysteine (GGC), which is the immediate precursor to glutathione, rapidly boosts cellular glutathione levels following a single dose in healthy individuals. Efficacy of GGC against oxidative stress induced by Pseudomonas aeruginosa, which is a common and chronic pathogen infecting lungs of CF patients, remains unassessed. Primary mucocilliary differentiated airway (bronchial and/or nasal) epithelial cells were created from four individuals with CF. Airway oxidative stress and inflammation was induced by P. aeruginosa lipopolysaccharide (LPS). Parameters including global proteomics alterations, cell redox state (glutathione, oxidative stress), pro-inflammatory mediators (IL-8, IDO-1), and cellular health (membrane integrity, stress granule formation, cell metabolic viability) were assayed under six experimental conditions: (1) Mock, (2) LPS-chal

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Author

Hewson, Chris K.; Capraro, Alexander; Wong, Sharon L.; Pandzic, Elvis; Zhong, Ling; Fernando, Bentotage S. M.; Awatade, Nikhil T.; Hart-Smith, Gene; Whan, Renee M.; Thomas, Shane R.; Jaffe, Adam; Bridge, Wallace J.; Waters, Shafagh A.

Publisher

MDPI AG; Multidisciplinary Digital Publishing Institute (MDPI); Basel: MDPI AG, 2012- (ISSN 2076-3921)

Published

2020

Language

EN

Field

Medicine (Health Sciences)