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Lysosomal autophagy promotes recovery in rats with acute knee injury through TFEB mediation by Xia, Qingquan; Wu, Xuhua; Rong, Ke; Zhou, Zhenyu; Li, Xujun; Fei, Teng; Yin, Xiaofan is a Medicine article available to read on EtoBox.
What is Lysosomal autophagy promotes recovery in rats with acute knee injury through TFEB mediation about?
## Background To study the role of lysosomal decomposition and elimination of old bone matrix, as well as the mechanism of promoting chondrocyte growth and bone recovery through the perspective of TFEB-mediated lysosomal autophagy. ## Methods Rat models of acute knee injury were designed, and autophagy flow was detected by injection of autophagy inhibitors 3-methyladenine. Autophagy flow was detected by RFP-GFP-LC3 double fluorescence molecule. The expression of TFEB, DRAM, MAPLC3, and MITF were analyzed by Western blot, and the expression of genes NITF, Bcl2, and TYR in rat cartilage tissues were detected by RT-PCR. ## Results The number of autophagosomes was increasing in the auto group compared with the inhibitor-auto group and normal group. There was a significant difference of LC3 levels in the auto group and inhibitor-auto group compared with the normal control. The expression of TFEB, DRAM, MAPLC3, and MITF proteins by Western blot analysis were significantly increased in the auto group and decreased in the inhibitor-auto group. The expression of NITF, Bcl2, and TYR by RT-PCR determination were higher in the auto group and inhibitor-auto group than the normal group. ## Concl
Who reads Lysosomal autophagy promotes recovery in rats with acute knee injury through TFEB mediation?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Xia, Qingquan; Wu, Xuhua; Rong, Ke; Zhou, Zhenyu; Li, Xujun; Fei, Teng; Yin, Xiaofan
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2006-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1749-799X)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)