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Off-line Elimination of Carbohydrates for Amino Acid Analysis of Samples with High Carbohydrate Content by Ion-exchange Chromatography by Yongsheng Ding; Hong Yu; Shifen Mou is a Chemistry article available to read on EtoBox.
What is Off-line Elimination of Carbohydrates for Amino Acid Analysis of Samples with High Carbohydrate Content by Ion-exchange Chromatography about?
This paper introduces a new off-line sample preparation that eliminates carbohydrates from amino acid samples containing a high carbohydrate content before analysis by anion-exchange chromatography and integrated pulsed amperometric detection. First, the sample is introduced into a cation-exchange column in the hydrogen form. Carbohydrates are removed completely using 0.02% formic acid as a transfer fluid, while only amino acids are retained. Amino acids are then extracted from the cation-exchange resin by 10 ml of 1 M ammonia. The ammonia collected is evaporated to dryness and the residue redissolved in water containing 20 mg/l NaN3 for injection. All amino acids are recovered following the carbohydrate removal step. The average recovery is 97.2%. The relative standard deviation for seven replicates is less than 5.2%. The usefulness of the method is illustrated with chromatograms of ratafia samples obtained before and after the off-line removal of carbohydrates.
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- Author
- Yongsheng Ding; Hong Yu; Shifen Mou
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2003
- Language
- EN
- Field
- Chemistry (Physical Sciences)