About this Engineering article

Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver by Libuse Trnkova; Sona Krizkova; Vojtech Adam; Jaromir Hubalek; Rene Kizek is a Engineering article available to read on EtoBox.

In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on the capturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals -labelled as cys MT and W a -were observed. The cys MT corresponded to -SH moieties of MT and W a corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 g/ml) were optimized to suggest a silver(I) ions biosensor. Biosensor (CPE modified with anti-MT antibody) prepared under the optimized conditions was then used for silver(I) ions detection. The detection limit (3 S/N) for silver(I) ions was estimated as 0.5 nM. The proposed biosensor was tested by detection spiking of silver(I) ions in various water samples (from very p

Author

Libuse Trnkova; Sona Krizkova; Vojtech Adam; Jaromir Hubalek; Rene Kizek

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2011

Language

EN

Field

Engineering (Physical Sciences)