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The DNA Fragmentation Index (DFI) test, known as the sperm chromatin dispersion Assay (SCD), assesses sperm DNA integrity by evaluating the formation of halos after DNA denaturation. The procedure involves preparing a semen sample, embedding sperm in agarose, and using specific staining solutions to visualize DNA fragmentation under a microscope. The DFI is calculated based on the percentage of sperm with fragmented DNA, which indicates genetic quality and fertility potential.

Author

twinklechavda1803

Language

EN