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A Porcine Ex Vivo Lung Perfusion Model To Investigate Bacterial Pathogenesis by Dumigan, Amy; Fitzgerald, Marianne; Santos, Joana Sá-Pessoa Graca; Hamid, Umar; OâKane, Cecilia M.; McAuley, Danny F.; Bengoechea, Jose A.; Allen, Irving Coy; Goldberg, Joanna B. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A Porcine Ex Vivo Lung Perfusion Model To Investigate Bacterial Pathogenesis about?

The implementation of infection models that approximate human disease is essential to understand infections and for testing new therapies before they enter into clinical stages. Rodents are used in most preclinical studies, although the differences between mice and humans have fueled the conclusion that murine studies are unreliable predictors of human outcomes. In this study, we have developed a whole-lung porcine model of infection using the ex vivo lung perfusion (EVLP) system established to recondition human lungs for transplant. As a proof of principle, we provide evidence demonstrating that infection of the porcine EVLP with the human pathogen Klebsiella pneumoniae recapitulates the known features of Klebsiella -triggered pneumonia. Moreover, our data revealed that the porcine EVLP model is useful to reveal features of the virulence of K. pneumoniae , including the manipulation of immune cells. Together, the findings of this study support the utility of the EVLP model using pig lungs as a surrogate host for assessing respiratory infections.

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Author

Dumigan, Amy; Fitzgerald, Marianne; Santos, Joana Sá-Pessoa Graca; Hamid, Umar; OâKane, Cecilia M.; McAuley, Danny F.; Bengoechea, Jose A.; Allen, Irving Coy; Goldberg, Joanna B.

Publisher

American Society for Microbiology; Washington D.C.: American Society for Microbiology (ISSN 2150-7511)

Published

2019

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)