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Regulation of intrinsic polarity establishment by a differentiation-type MAPK pathway in S. cerevisiae by Prabhakar, Aditi; Chow, Jacky; Siegel, Alan J.; Cullen, Paul J. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Regulation of intrinsic polarity establishment by a differentiation-type MAPK pathway in S. cerevisiae about?

All cells establish and maintain an axis of polarity that is critical for cell shape and progression through the cell cycle. A well-studied example of polarity establishment is bud emergence in yeast, which is controlled by the Rho GTPase Cdc42p. The prevailing view of bud emergence does not account for regulation by extrinsic cues. Here, we show that the filamentous growth MAPK pathway (fMAPK) regulates bud emergence under nutrient-limiting conditions. The fMAPK pathway regulated the expression of polarity targets including a direct effector of Cdc42p, Gic2p. The fMAPK pathway also stimulated GTP-Cdc42p levels, which is a critical determinant of polarity establishment. The fMAPK pathway activity was spatially restricted to bud sites and active during the period of the cell cycle leading up to bud emergence. Time-lapse fluorescence microscopy showed that the fMAPK pathway stimulated the rate of bud emergence during filamentous growth. Unregulated activation of the fMAPK pathway induced multiple rounds of symmetry breaking inside the growing bud. Collectively, our findings identify a new regulatory aspect of bud emergence that sensitizes this essential cellular process to external c

Who reads Regulation of intrinsic polarity establishment by a differentiation-type MAPK pathway in S. cerevisiae?

Author

Prabhakar, Aditi; Chow, Jacky; Siegel, Alan J.; Cullen, Paul J.

Publisher

The Company of Biologists Ltd; The Company of Biologists; The Company of Biologists Ltd. (ISSN 0021-9533)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)